restriction analysis

[citroenboom]

If your PCR is successful (check 5 ul on 0.8% agarose after the run) you can load the whole PCR mix on gel and cut it out. I prefer to use PCR-purification kit, but both works. So, yes, I think your procedure should work fine.

[JackBean]

sid: That's depends, whether is the PCR only to confirm that you have the gene cloned into vector or whether you want to do with the PCR amplicon something subsequently. I would bet on the first option, because pPICZ is expression vector.

[siddharthsameer]

hello
I am about to start and my first step would be the amplification of my desired gene.. and then digestiona nd ligation into expression vector.. so If i start from the first step... thanks you so mucg for clearing my doubts...