Login

Join for Free!
117006 members


HL60 cells centrifugation

Discussion of all aspects of cellular structure, physiology and communication.

Moderator: BioTeam

HL60 cells centrifugation

Postby claire84 » Wed Jul 04, 2012 3:58 pm

Hi everyone!
I need your help because I have little experience on cell culture techniques...I would centrifuge my Hl60 cells which are plated in a 96-well microplate, to obtain a pellet at the bottom and to remove supernatant without loss of cells.
Could you recommend me rpm and time to use?
I tryed with 1500 rpm for 5 minutes, but I think I need to increase the time...
thank so much

Chiara
claire84
Garter
Garter
 
Posts: 13
Joined: Mon Aug 23, 2010 1:51 pm

Postby JackBean » Thu Jul 19, 2012 6:44 am

5000 g for 1 minute is used for bacteria and I don't think it would be much different for other cells.
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.
User avatar
JackBean
Inland Taipan
Inland Taipan
 
Posts: 5667
Joined: Mon Sep 14, 2009 7:12 pm


Return to Cell Biology

Who is online

Users browsing this forum: No registered users and 1 guest

cron