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DNA extraction from Human blood

Discussion of all aspects of biological molecules, biochemical processes and laboratory procedures in the field.

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DNA extraction from Human blood

Postby tranglee » Wed Jul 04, 2012 2:42 am

i extracted DNA from human blood by phenol. i think this result is not good, but i don't known clearly, i tried many time but still the same, can you give some opinion. please, see the image I show in here. thank you very much.
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DNA production run electrophoresis gel 0.8%
tranglee
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Postby canalon » Wed Jul 04, 2012 3:21 am

First you need an orange filter between your camera and your gel. It will tremendously improve your image.

Second, in absence of any reference (Molecular weight marker or anything else, or even explanation of how the gel was run it is hard to say how bad (or not) your result is.
Patrick

Science has proof without any certainty. Creationists have certainty without
any proof. (Ashley Montague)
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Postby triton » Thu Sep 13, 2012 11:12 am

DNA looks good. There is some problem with electrophoresis. Probably you overloaded (too much DNA) and thus the wierd appearance of your bands. Try to load little bid less of sample and use lower voltage. stain the gel after electrophoresis, not before.
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Postby Cat » Fri Sep 14, 2012 2:58 pm

1. Too much Ethidium Bromide.
2. If this is supposed to be total DNA, it's heavily degraded. Check for contamination...
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