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Can I deduce the Number of Proteins Produced?

Genetics as it applies to evolution, molecular biology, and medical aspects.

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Can I deduce the Number of Proteins Produced?

Postby blah484 » Thu Jun 28, 2012 3:53 pm

I have a question given to me, reading:

Calculate the number of proteins with an average molecular weight of 90,000 Da that are coded by a
genome assuming that 80% of the a genome contain open reading frames.

The average molecular weight of an amino acid is 50 Da and that every amino acid is coded by 3 nucleotides, and each
open reading frame ends by a stop codon that doesn't code for any AA.

I know that: The length of the Genome is 9.7 x 10^6 base pairs

How do I calculate the number of proteins?
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Postby JackBean » Fri Jun 29, 2012 7:11 am

Are you sure it should be 50 Da? Even Gly has 75 Da :-/ http://www.wolframalpha.com/input/?i=av ... amino+acid

Anyway, after you get the right number, just calculate, how many amino acids average protein contains. From this you get the average number of nucleotides (do not forget the STOP codons). And since you know, that 80% of 9.7 x 10^6 base pares codes for proteins, it should not be that hard to calculate number of average proteins.
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