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Flow cytometry-antibody conc.

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Flow cytometry-antibody conc.

Postby biology_06er » Sat Jun 23, 2012 4:22 am

Hi there,

I'll be doing FC for the first time next week, and I have a protocol but I'm slightly confused about one thing...after I resuspend my cells in PBS/BSA it tells me to take 200uL of cells and incubate with 1:200 of primary antibody...so does this mean if I have 200uL of cells, I add 1uL of my antibody...and then if I wanted to to another conc of antibody say 1:400 I would add 0.5uL of antibody...I recall being told I would have to do serial dilutions of my primary antibody..but how do I go about doing serial dilutions..if i need 200uL of cells to start with?

Hope I make sense!
cheers,
b_06er
biology_06er
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Postby biology_06er » Sun Jun 24, 2012 11:14 am

Problem solved!
biology_06er
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