Culturing cyanobacteria - urgent advice please!!

[JMJABT]

Hello,

I am currently trying to start a series of trials using Oscillatoria tenuis, Anabaena circinalis and Planktothrix aghardiulture the. I have obtained my cutures from CCAP in Cambridge, and am starting to try to culture them. However, I can find very little information on-line on culture techniques, advice, etc.

Three weeks ago, I inoculated the cultures at a ratio of 1:10 with UTEX medium as advised by CCAP. I was not sure on light levels so I put them in a slightly shaded window with very gentle aeration.

After two weeks I placed them on a light bench with around 5000 lux of standard white fluorescent light (this as the only value I could find on-line). I have since removed the aeration in case this was having an effect. I now have very slightly green water, a little cliudy, but no major growth. I was advised that after 4 weeks I should inoculate again at 1:10 when there was a dense culture. I am nearly at that point and I cant't see any filaments or anything of the sort.

I really need someone's help as this is an expensive project, and they are expensive cultures, and I cant get them to do anything... If required I can add pictures.

Thanks in advance,

Jack

[JackBean]

there are no articles about these cyanobacteria at PubMed at all?

[JMJABT]

There are a few, but I don't have access to most fulltext articles, and therefore they are of limited use as I cant access the methodologies...

[JackBean]

Then try to ask for the articles. Either the authors or someone you know (or don't).