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sample(material) needed from a person to genome sequence?

Genetics as it applies to evolution, molecular biology, and medical aspects.

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sample(material) needed from a person to genome sequence?

Postby GeneticEngineer » Wed May 30, 2012 4:59 am

What do you need to obtain from a person to perform genome sequence test? like what bodily biological material? like hair,saliva,blood,reproductive fluid etc..
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Postby JackBean » Wed May 30, 2012 6:09 am

anything that contains genomic DNA
http://www.biolib.cz/en/main/

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Postby DougB » Wed May 30, 2012 4:24 pm

We find buccal (cheek) swabs to be the easiest to work with for genomic DNA. Only hair root has genomic DNA. Blood and reproductive fluid tends to be more difficult to obtain. You can get a decent yield of quality DNA from the buccal swabs.
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Postby GeneticEngineer » Wed May 30, 2012 5:09 pm

The type of sequencing im looking at is called Whole genome sequencing/ full genome sequencing. I heard you need something called plasmid to do it. How do you get that from someone?
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Postby JackBean » Wed May 30, 2012 6:10 pm

You do not get plasmid from anyone. You get some cells, isolate the gDNA, break into pieces, insert into plasmids (which you provide) and then do the sequencing.
However, with next-gen sequencing you do not need to put the gDNA into vectors, but sequence it immediately.
http://www.biolib.cz/en/main/

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Re: sample(material) needed from a person to genome sequence?

Postby GeneticEngineer » Wed May 30, 2012 11:19 pm

My question really is: what is the best body tissue/fluid type to get the cells from?
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Postby JackBean » Thu May 31, 2012 5:51 am

as DougB wrote, it is easiest to take buccal swabs, other option is hair, if you have them with hair roots, or blood.
http://www.biolib.cz/en/main/

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