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sample(material) needed from a person to genome sequence?Moderator: BioTeam
7 posts • Page 1 of 1
sample(material) needed from a person to genome sequence?What do you need to obtain from a person to perform genome sequence test? like what bodily biological material? like hair,saliva,blood,reproductive fluid etc..
The type of sequencing im looking at is called Whole genome sequencing/ full genome sequencing. I heard you need something called plasmid to do it. How do you get that from someone?
You do not get plasmid from anyone. You get some cells, isolate the gDNA, break into pieces, insert into plasmids (which you provide) and then do the sequencing.
However, with next-gen sequencing you do not need to put the gDNA into vectors, but sequence it immediately. http://www.biolib.cz/en/main/
Cis or trans? That's what matters.
Re: sample(material) needed from a person to genome sequence?My question really is: what is the best body tissue/fluid type to get the cells from?
7 posts • Page 1 of 1
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