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Idea to determine order of assembly of spliceosome

Discussion of all aspects of biological molecules, biochemical processes and laboratory procedures in the field.

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Postby JackBean » Thu May 31, 2012 8:48 am

MS would be better, because it will identify the proteins, if you do not know them. If you do not know the proteins, you can hardly have antibody and without antibody it would be difficult to do Western blot ;)

The phage promoters should ensure strong expression in bacteria, because that's what phage wants - to express its genes ;)
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.
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Postby nukeman » Thu May 31, 2012 4:52 pm

I am aware of that - luckily I'm allowed to modify the question the way I want. :P It's not so much that we should get a "right/wrong" answer, it's more about the teacher evaluating the use of our knowledge and methodical approach.
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Postby JackBean » Fri Jun 01, 2012 7:38 am

But in such case we can go back to my first post...
http://www.biolib.cz/en/main/

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Postby nukeman » Fri Jun 01, 2012 2:14 pm

Yeah, I did something like that. Thought I'd look around what other options were available, never hurts to know more :P

Anyways, thank you very much for your help :)
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