Biology-Online • View topic - how to make blood lysis buffer
Login

Join for Free!
121820 members
Advertisement
Advertisement

how to make blood lysis buffer

Discussion of all aspects of biological molecules, biochemical processes and laboratory procedures in the field.

Moderator: BioTeam

how to make blood lysis buffer

Postby karenzellie » Thu May 24, 2012 5:12 pm

Hi,

I was wondering how I should make this recipe:

100mM Tris HCL
100mM EDTA
100mM Nacl
1%SDS
karenzellie
Garter
Garter
 
Posts: 2
Joined: Thu May 24, 2012 5:08 pm

Postby JackBean » Fri May 25, 2012 5:14 am

What's the problem?
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.
User avatar
JackBean
Inland Taipan
Inland Taipan
 
Posts: 5694
Joined: Mon Sep 14, 2009 7:12 pm

Re: how to make blood lysis buffer

Postby karenzellie » Fri May 25, 2012 2:48 pm

I know i need to make stock solutions of the buffers but how do I make this into a solution together to make the appropriate concentrations of each chemical?
karenzellie
Garter
Garter
 
Posts: 2
Joined: Thu May 24, 2012 5:08 pm


Postby JackBean » Fri May 25, 2012 3:23 pm

You can make 1M Tris, 1M EDTA, 1M NaCl and 10% SDS, mix 100 ml of each and fill up to 1 l.
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.
User avatar
JackBean
Inland Taipan
Inland Taipan
 
Posts: 5694
Joined: Mon Sep 14, 2009 7:12 pm


Return to Molecular Biology

Who is online

Users browsing this forum: No registered users and 0 guests