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In search of genomes

Genetics as it applies to evolution, molecular biology, and medical aspects.

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In search of genomes

Postby Ejager » Thu May 10, 2012 12:43 pm

For a high school biology project we’re ''investigating'' the DNA of the Drosophila melanogaster.
Our assignment is to make a lab for fellow students to find/show the genetic difference between two types of Drosophila. One mutant with white eyes and one ‘’normal’’ with red eyes.

We wanted to use PCR and then gel electrophoresis

Now, our biology teacher is not a particular expert on this subject and we have some questions. Hoped you could help.

First of all, we need to find the right genomes. We tried Flybase, but with our limited knowledge we didn’t find any useful information. And then, how can we find the sequence of those genomes so we could find the right primer for the PCR?

All tips are very welcome, we really hope you could help us!
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Postby JackBean » Thu May 10, 2012 1:47 pm

For this you need to find sequence of the gene coding for the color of eyes. I think it was mentioned in Alberts' The cell, which gene is responsible.
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.
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