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Isolation during transcription

Discussion of all aspects of biological molecules, biochemical processes and laboratory procedures in the field.

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Isolation during transcription

Postby Trenchent » Fri May 04, 2012 8:35 am

Polio and rotoviruses use polymerase in their transcription process.

You need to stop the viruses from using the transcription enzymes by stopping transcription before the binding

you need to bind to the single helix amino acids before the polymerase does

You need a technique to do this which won't destroy the enzymes


Then the cell will decompose as it is only half formed

The transcription enzymes will be left in the solution and easily extractable by centrifuge

The more transcription about to take place, interrupted the higher the yield of polymerase


then you need a technique to synthesis telomerase from the polymerase


good luck
please post test results and compounds as replys
we are doing this for humanity to become immortals

be a bit altruistic

It took me 25 seconds to double check that i wrote this properly. 50 people died in the world during the same time. WE need to work together to get this done as fast as possible. How many people would you kill through neglect?

2 parts nitrogen one part lycosene... possibly
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Postby JackBean » Fri May 04, 2012 8:41 am

OK, I will ask only once, next time I will ban you immediately.

What the hell?
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.
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Re: Isolation during transcription

Postby AstraSequi » Fri May 04, 2012 7:12 pm

Trenchent wrote:Polio and rotoviruses use polymerase in their transcription process.

You need to stop the viruses from using the transcription enzymes by stopping transcription before the binding

you need to bind to the single helix amino acids before the polymerase does

You need a technique to do this which won't destroy the enzymes


Then the cell will decompose as it is only half formed

The transcription enzymes will be left in the solution and easily extractable by centrifuge

The more transcription about to take place, interrupted the higher the yield of polymerase

If all you want to do is isolate some RNA polymerase, there are far easier ways to do it (and which don't involve viruses). In fact, some of these polymerases are sold commercially.


then you need a technique to synthesis telomerase from the polymerase

If you want to make telomerase in vitro, you will first need to determine all of the genes that code for the components that make up the telomerase enzyme (it is a multi-subunit complex), the chemical modifications that are required for its activity, and how to successfully assemble the components. This is not known. If you want to use it as a drug, you will also have to figure out how to deliver it intact to your cells without getting destroyed, and then how to get it to the nucleus. All of these steps are non-trivial.


good luck
please post test results and compounds as replys
we are doing this for humanity to become immortals

be a bit altruistic

This is a worthy goal, but telomerase alone is highly unlikely to do it. The aging process is far more complex than that, and furthermore telomere-induced senescence is an important anti-cancer mechanism.
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