Biology-Online • View topic - Artificial phosphorylation by using different detergents
Login

Join for Free!
121731 members
Advertisement
Advertisement
Advertisement


Artificial phosphorylation by using different detergents

Discussion of all aspects of cellular structure, physiology and communication.

Moderator: BioTeam

Artificial phosphorylation by using different detergents

Postby MrsHappyy » Tue Apr 17, 2012 6:19 am

Is it possible that using of different detergents (for isolation of lipid microdomains) has any influence on different phosphorylation of proteins in signaling pathways? Thanks for your anwers
MrsHappyy
Garter
Garter
 
Posts: 2
Joined: Tue Apr 17, 2012 5:45 am

Postby JackBean » Tue Apr 17, 2012 6:54 am

It will affect which proteins you will extract, thus you may get different pattern, although the material will be the same.
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.
User avatar
JackBean
Inland Taipan
Inland Taipan
 
Posts: 5694
Joined: Mon Sep 14, 2009 7:12 pm

Re: Artificial phosphorylation by using different detergents

Postby MrsHappyy » Tue Apr 17, 2012 8:04 am

Thanks :-) And is it possible that some signaling pathways, which are investigated, can be described with mistakes due to using of different detergents :shock: ? Is there any publication about it?
MrsHappyy
Garter
Garter
 
Posts: 2
Joined: Tue Apr 17, 2012 5:45 am



Return to Cell Biology

Who is online

Users browsing this forum: No registered users and 1 guest

Advertisement