Login

Join for Free!
119272 members


Hard Question

Discussion of all aspects of biological molecules, biochemical processes and laboratory procedures in the field.

Moderator: BioTeam

Hard Question

Postby mxnick262 » Wed Apr 04, 2012 7:57 pm

You have performed yeast two hybrid assay to identify proteins that interact with superman and identified a gene coding for kryptonite that interacts with superman. What experiments you will perform to demonstrate that the superman interacts with kryptonite in-vivo (inside nucleus in the cell)?

Give atleast two examples.
mxnick262
Garter
Garter
 
Posts: 15
Joined: Wed Apr 04, 2012 6:54 pm

Postby JackBean » Thu Apr 05, 2012 2:08 pm

Where is your attempt to solve this "hard" problem? Have you not done any such methods in your course?
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.
User avatar
JackBean
Inland Taipan
Inland Taipan
 
Posts: 5692
Joined: Mon Sep 14, 2009 7:12 pm

Postby mxnick262 » Tue Apr 17, 2012 1:28 am

Please post something mildly useful. Methods that I have considered: protein-complex immunoprecipitation coupled with the use of some sort of molecular beacon? Guidance please.
mxnick262
Garter
Garter
 
Posts: 15
Joined: Wed Apr 04, 2012 6:54 pm


Postby JackBean » Tue Apr 17, 2012 6:53 am

I thought you were asked for in vivo method, not in vitro
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.
User avatar
JackBean
Inland Taipan
Inland Taipan
 
Posts: 5692
Joined: Mon Sep 14, 2009 7:12 pm

Postby mxnick262 » Tue Apr 17, 2012 3:54 pm

Yea you are right. This question has me stumped.
mxnick262
Garter
Garter
 
Posts: 15
Joined: Wed Apr 04, 2012 6:54 pm

Postby mxnick262 » Tue Apr 17, 2012 11:23 pm

BiFC. It's really a hypothetical question so even though this method has absolutely nothing to do with what we have learned this semester, it may still be right.

Still need one more method. GFP is to similar...
mxnick262
Garter
Garter
 
Posts: 15
Joined: Wed Apr 04, 2012 6:54 pm

Postby mxnick262 » Tue Apr 17, 2012 11:33 pm

FRET*
mxnick262
Garter
Garter
 
Posts: 15
Joined: Wed Apr 04, 2012 6:54 pm

Postby JackBean » Wed Apr 18, 2012 6:25 am

Here you go, you have answered your question by your own.
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.
User avatar
JackBean
Inland Taipan
Inland Taipan
 
Posts: 5692
Joined: Mon Sep 14, 2009 7:12 pm


Return to Molecular Biology

Who is online

Users browsing this forum: Exabot [Bot] and 1 guest