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Help identifying unknown bacteria

About microscopic forms of life, including Bacteria, Archea, protozoans, algae and fungi. Topics relating to viruses, viroids and prions also belong here.

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Help identifying unknown bacteria

Postby anthony7 » Sun Apr 01, 2012 9:21 pm

Hell everyone, I am trying to identify my unknown bacteria
Gram negative- I believe rod shaped
strict aerobe-thioglycollate test, although the deep stab appeared to be fac. anaerobic I might have screwed up the deep stab
slow urea hydrolysis, weak urease prod-urea agar
citrate utilization-citrate test
no glucose fermentation, degradation of peptone and alkaline end products with sucrose and lactose fermentation test-Phenol Red tests
EMB test- gram negative
SIM test- tryptophan broken down, indole positive, no sulfur reduction
decarboxylation tests- decarboxylation- org has enzymes to break down lysine, arginine, and ornithine
catalase test- catalase positive
I was thinking my organisms could be pseudomonas aeroginosa,
ANy advice would be greatly appreciated
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Re: Help identifying unknown bacteria

Postby JorgeLobo » Sun Apr 01, 2012 10:19 pm

Need oxidase but maybe not if indole +. Any pigmentation, odor?
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Postby anthony7 » Sun Apr 01, 2012 10:32 pm

it was hard to decifer between a pink shade on the EMB plate, I felt like it was clear which means gram (-), I dont remember a specific odor, we didnt run a oxidase test there were only about 10 specific tests we ran
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Postby anthony7 » Sun Apr 01, 2012 10:34 pm

no specific color on the nutrient plate I think i was debating on pseudomonas, shigella, moraxella
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Postby anthony7 » Mon Apr 02, 2012 12:37 am

I believe my organism could be Alcaligenes faecalis or pseudomonas aerugenosa .....any thoughts????
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Postby JorgeLobo » Mon Apr 02, 2012 12:49 pm

Do an oxidase and check motility.
Was citrate pos or neg?
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Postby anthony7 » Tue Apr 03, 2012 1:56 am

strict aerobe by thioglyoclate test, negative carb fermentation, positive citrate, negative indole, positive urease, catalase positive, gram negative rod,positive decarboxylation tests......I think I have Alcaligenes faecalis or pseudomonas aerugenosa. Motility was positive from the deep stab. I think my test results match those organism except for the positive urease test
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Postby anthony7 » Tue Apr 03, 2012 2:03 am

We couldnt run additional tests but if we could have I would have ran a gelatinase or nitrate reduction test to differentiate between the two organisms.
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Re: Help identifying unknown bacteria

Postby JorgeLobo » Tue Apr 03, 2012 11:06 am

Can;t say much for your prof in designing this lame id study that ignores the standard flow of ID that takes you through the more consistent and basic considerations.
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