Help identifying unknown bacteria

[anthony7]

Hell everyone, I am trying to identify my unknown bacteria
Gram negative- I believe rod shaped
strict aerobe-thioglycollate test, although the deep stab appeared to be fac. anaerobic I might have screwed up the deep stab
slow urea hydrolysis, weak urease prod-urea agar
citrate utilization-citrate test
no glucose fermentation, degradation of peptone and alkaline end products with sucrose and lactose fermentation test-Phenol Red tests
EMB test- gram negative
SIM test- tryptophan broken down, indole positive, no sulfur reduction
decarboxylation tests- decarboxylation- org has enzymes to break down lysine, arginine, and ornithine
catalase test- catalase positive
I was thinking my organisms could be pseudomonas aeroginosa,
ANy advice would be greatly appreciated

[JorgeLobo]

Need oxidase but maybe not if indole +. Any pigmentation, odor?

[anthony7]

it was hard to decifer between a pink shade on the EMB plate, I felt like it was clear which means gram (-), I dont remember a specific odor, we didnt run a oxidase test there were only about 10 specific tests we ran

[anthony7]

no specific color on the nutrient plate I think i was debating on pseudomonas, shigella, moraxella

[anthony7]

I believe my organism could be Alcaligenes faecalis or pseudomonas aerugenosa .....any thoughts????

[JorgeLobo]

Do an oxidase and check motility.
Was citrate pos or neg?

[anthony7]

strict aerobe by thioglyoclate test, negative carb fermentation, positive citrate, negative indole, positive urease, catalase positive, gram negative rod,positive decarboxylation tests......I think I have Alcaligenes faecalis or pseudomonas aerugenosa. Motility was positive from the deep stab. I think my test results match those organism except for the positive urease test

[anthony7]

We couldnt run additional tests but if we could have I would have ran a gelatinase or nitrate reduction test to differentiate between the two organisms.

[JorgeLobo]

Can;t say much for your prof in designing this lame id study that ignores the standard flow of ID that takes you through the more consistent and basic considerations.