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HELP! Cannot decide which Gram+, rod-shaped organism this is

About microscopic forms of life, including Bacteria, Archea, protozoans, algae and fungi. Topics relating to viruses, viroids and prions also belong here.

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HELP! Cannot decide which Gram+, rod-shaped organism this is

Postby Kat000 » Sat Mar 31, 2012 11:32 pm

I've boiled it down to it either being Bacillus megaterium, Bacillus subtilis, Clostridium sporogenes, Corynebacterium pseudodiptherium, or Lactobacillus acidophilus.

My organism is motile, acid litmus milk,tested postive for Methyl Red, Voges-Proskauer, Citrate, Catalse tests, and tested negative for Oxidase, Urease, H2S production and sucrose and mannitol tests.

For the test of fermentation glucose, my organism was acid, and gas was produced...

PLEASE PLEASE PLEASE help me! Thanks!
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Re: HELP! Cannot decide which Gram+, rod-shaped organism this is

Postby JorgeLobo » Sun Apr 01, 2012 12:29 am

Think you'd better try that Gram stain again. Among the species you offered is an anaerobic bacterium, one that grows poorly if at all on media neutral in pH, more than one that's nonmotile and a number that produce obvious spores.
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Postby Kat000 » Sun Apr 01, 2012 12:42 am

My professor said it was GRAM POSITIVE, she corrected me after I thought it was Gram Negative...
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Re: HELP! Cannot decide which Gram+, rod-shaped organism this is

Postby JorgeLobo » Sun Apr 01, 2012 1:24 pm

What IS the Gram reaction? Maybe your prof forget to tell the isolate what it's supposed to be.
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