Subcellular Fractionation question

[testsubjectzz]

You're going to get 4 fractions when you're doing this

Cytosolic proteins
Membrane proteins
Nuclear Proteins
Cytoskeletal proteins

For example in the Qproteome kit it breaks open the plasma membrane but doesn't solubilise it you spin that down the supernatant are the cytosolic proteins but once you've broken the plasma membrane your organelles for example the ER and mitochondria will also be disrupted?

Also are mitochondrias free floating within the cell or are they connected via cytoskeletal proteins?

[JackBean]

They should be disrupted when you add little of deterged (but less than for solubilization of membranes)