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Antibody Reconstitution

Discussion of all aspects of biological molecules, biochemical processes and laboratory procedures in the field.

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Antibody Reconstitution

Postby knclayton » Sun Feb 19, 2012 9:36 pm

Hi All,

I have question regarding some antibodies I wish to reconstitute.

The instruction form that came with the order states, "Reconstitute with 0.1 ml sterile distilled water (Final concentration=1mg/ml. Final buffer = 0.01 M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide)"

So, does this mean I just use sterile distilled water, or am I somehow supposed to use PBS/BSA/etc., or is the PBS/BSA/etc. just part of the current makeup of the antibody vial that I have purchased?

Thanks!
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Postby canalon » Mon Feb 20, 2012 3:03 am

Sounds to me like thr PBS/BSA/... are in the vial, and that by adding the H2O you will have the solution described.
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