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## Problem with Lab result

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### Problem with Lab result

So I need help on how to do this graph correctly:

The experiment is Enumeration of Bacteria:The standard plate count

My results for the Quantitative plating method is:
Dilution Bottle --- ml Plated --- Dilution --- Dilution Factor --- Number of colonies
b(1:10,000) --- 1.0 --- 1:100,000 --- 10^4 --- TMC
b(1:10,000) --- 0.1 --- 1:1,000,000 --- 10^5 --- TMC
c(1:1,000,000) --- 1.0 --- 1:1,000,000 --- 10^6 --- 267
c(1:1,000,000) --- 0.1 --- 1:10,000,000 --- 10^7 --- 31

b) how many cells per ml were in the undiluted culture?___I didn't get this answer

Second Part:
Optical density determination

Dilution --- Optical Density
1:1 --- 30% T; 0.56 A
1:2 --- 50% T ; 0.33 A
1:4 --- 72% T; 0.16 A
1:8 --- 85% T; 0.09 A
1:16 --- 91% T; 0.05 A

The graph x-axis is Dilution & the y is Optical Density. What number or calculation am I suppose to do to get the optical density because if I use A in optical density my graph goes down negative I dont think it's correct. Please help. Thank you.
Layd33foxx
Garter

Posts: 21
Joined: Tue Feb 14, 2012 4:31 am

a) If you get 267 colonies with 10^6 dilution, the original number is 267.10^6; if you get 31 colonies with dilution 10^7, the original number was 31.10^7. Now just make average of these two numbers.

Was that so hard?

b) the more cells, the more light is dispersed and thus less light is passed through. Thus when you dilute your sample, you have less bacteria per volume and thus the optical density decreases.
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.

JackBean
Inland Taipan

Posts: 5694
Joined: Mon Sep 14, 2009 7:12 pm

Thank you for clearing it up
Layd33foxx
Garter

Posts: 21
Joined: Tue Feb 14, 2012 4:31 am

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