Login

Join for Free!
111304 members


Question about Kirby-Bauer method

About microscopic forms of life, including Bacteria, Archea, protozoans, algae and fungi. Topics relating to viruses, viroids and prions also belong here.

Moderator: BioTeam

Question about Kirby-Bauer method

Postby miramax » Fri Feb 10, 2012 5:15 am

Hi, I'm trying to do the Kirby-Bauer method by testing the effect of certain foods' ability to inhibit pathogen growth.

I was wondering if it's possible to do this method by just streaking the agar plates with pathogens like E.Coli straight from the tube (from the catalogue) or is it a must to use only pathogens after it has been grown in broth??

My plan was to just streak like E. Coli with an inoculating loop in a zig-zag pattern every 90 degrees, pretty much covering the whole plate. And then I would place my paper disks on top of the agar...

Thanks for replies.
miramax
Garter
Garter
 
Posts: 1
Joined: Fri Feb 10, 2012 5:08 am

Postby JackBean » Fri Feb 10, 2012 7:41 am

Usually you do overnight culture first. First, you will make sure the bacteria are viable. Second, you don't want to run out of your stock and order it again ;)
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.
User avatar
JackBean
Inland Taipan
Inland Taipan
 
Posts: 5624
Joined: Mon Sep 14, 2009 7:12 pm


Return to Microbiology

Who is online

Users browsing this forum: No registered users and 3 guests

cron