Login

Join for Free!
118243 members


Why RT-PCR

Discussion of all aspects of biological molecules, biochemical processes and laboratory procedures in the field.

Moderator: BioTeam

Why RT-PCR

Postby charisma » Sun Jan 29, 2012 4:26 pm

Hi,
I've done gene transformation to rice.To confirm that new gene has been transferred to rice I've already done genomic DNA PCR and the result showed the existence of the new gene but My supervisor asked me to do RT-PCR (Reverse transcription) too. Could someone please let me know what are RT-PCR preferences to genomic DNA? When I got the desire result by total genomic PCR, what's the point to do RT-PCR?
Thanks.
charisma
Garter
Garter
 
Posts: 1
Joined: Sun Jan 29, 2012 4:23 pm

Postby canalon » Sun Jan 29, 2012 4:57 pm

To see whether your gene is expressed, I would guess.
Patrick

Science has proof without any certainty. Creationists have certainty without
any proof. (Ashley Montague)
User avatar
canalon
Inland Taipan
Inland Taipan
 
Posts: 3909
Joined: Thu Feb 03, 2005 2:46 pm
Location: Canada

Postby JackBean » Mon Jan 30, 2012 9:46 am

yep, your gene may be silenced after incorporation or your promoter may not work properly, thus you need to check expression of your transgene.
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.
User avatar
JackBean
Inland Taipan
Inland Taipan
 
Posts: 5678
Joined: Mon Sep 14, 2009 7:12 pm



Return to Molecular Biology

Who is online

Users browsing this forum: No registered users and 4 guests