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dot blot--protoplasts

Discussion of all aspects of biological molecules, biochemical processes and laboratory procedures in the field.

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dot blot--protoplasts

Postby biology_06er » Wed Jan 11, 2012 1:35 am

A question regarding protoplasts in a dot blot..my protein of interest is in the protoplast which I has isolated and I have been told to do a titration/dilution of it...so I was told do 4x 10 fold diltuin...does this mean if I started with 1:10 my next wld be 1:100, 1:1000, 1:10000/---is this right? Is there a “certain” starting dilution I should start with?

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Postby JackBean » Wed Jan 11, 2012 7:12 am

the first one will be probably 1 : 1 ;)
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.
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