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E.coli Genotype

Discussion of all aspects of biological molecules, biochemical processes and laboratory procedures in the field.

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E.coli Genotype

Postby Kiki11 » Thu Dec 15, 2011 10:25 am

Hi,

I am trying to generate a minimal media for the E.coli strain with the following genotype
F'[lacIq Tn10(tetR)] mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 recA1 araD139 Δ(ara-leu)7697 galU galK rpsL(StrR) endA1 nupG
with the addition of Leucine I have managed to grow them, but the yield is anything but great. I am a little confused about the combination of araD139 and Δ(ara-leu) in the genotype.

araD139 means, that a mutation in L-ribulose-phosphate 4-epimerase blocks arabinose metabolism and
Δ(ara-leu) means, that the ability to built arabinose (and leucine) is deleted.

Can somebody help me and tell me if or if not my E.colis need arabinose? And/or if they are missing something else, please?

Thank you
Kiki
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Postby JackBean » Thu Dec 15, 2011 10:43 am

just try to add the arabinose and you will see ;)
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.
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Re: E.coli Genotype

Postby kdean » Tue Nov 06, 2012 4:18 pm

I believe that the ∆(ara, leu) simply means that your strain of bacteria cannot catabolize arabinose or leucine.
http://en.wikipedia.org/wiki/Bacterial_ ... menclature
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Postby JackBean » Tue Nov 06, 2012 9:17 pm

catabolise or synthesise?
http://www.biolib.cz/en/main/

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Re: E.coli Genotype

Postby citroenboom » Wed Nov 14, 2012 1:32 pm

According to openwetware it blocks metabolism.
I am using TOP10 and it has the same deletion. My cells do happily grow on M9 or the minimal medium of our group with leucine. DH5a doesn't have it I think.
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