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Ploblem in RNA 260/230 ratio

Discussion of all aspects of biological molecules, biochemical processes and laboratory procedures in the field.

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Ploblem in RNA 260/230 ratio

Postby ladilov » Sun Dec 11, 2011 10:49 pm

Hello everyone!
I isolated RNA from clam tissues recently to perform a Real Time PCR. I did using the TRIzol protocol and when I quantified the samples some of them have a 260/230 ratio as low as 0.30!!! I made an RNA clean-up using the RNaesy Mini from QIAGEN but they did not improve that much and I lost a considerable amount of sample. What can I do to get a better ratio without loosing sample?
ladilov
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