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yeast expression vector

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yeast expression vector

Postby fcs3 » Thu Dec 08, 2011 11:12 pm

Hi All,
I’m looking for a vector for expression of a third bridging protein in the Invitrogen Proquest yeast two-hybrid system.

Does anyone have or know of a yeast expression vector that suits the following requirements:
• High expression promoter that is independent of GAL system.
• Marker for selection of transformants in yeast that is not Leu, Trp, His or Ura.

I currently have clones set up with the Invitrogen two-hybrid system to test specific protein interactions, but I’ve found no interactions and recent publications have shown a third protein acts as a bridging protein for homologs in other species, so I’d like to try adding this protein in to my existing clones.
The yeast strain is S. cerevisiae Mav203 [MATα, leu2-3,112, trp1-901, his3Δ200, ade2-101, gal4Δ, gal80Δ, SPAL10::URA3, GAL1::lacZ, HIS3UAS GAL1::HIS3@LYS2, can1R, cyh2R].

Thanks in advance for your help!
fcs3
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Postby JackBean » Fri Dec 09, 2011 7:16 am

I'm sure, that if you try to search for S. cerevisiae on Invitrogen web site, you will find what are you looking for ;)
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.
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