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seeking opinion on an unknown

About microscopic forms of life, including Bacteria, Archea, protozoans, algae and fungi. Topics relating to viruses, viroids and prions also belong here.

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seeking opinion on an unknown

Postby sully325 » Tue Dec 06, 2011 6:11 pm


I am performing many biochemical tests for a morphological unknown. I am filling out the dichotomous key and would just like opinions as to if I am on the right track.


So far, I have:

Gram Negative Rods
Motility negative
MR-VP positive
Citrate positive
Indole Positive

My choices are :
C freundii
E aerogenes
S flexneri
K pneumonia
P vulgaris
S enteriditis

I am leaning towards E. aerogenes as it is fitting well with the above tests according to Bergey's manual. Anything else I can perform?
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Postby Cat » Sat Dec 10, 2011 7:12 pm

I would suggest you repeat your tests. According to your results it's not any of your choices.

C freundii is out as it's indole -ve.
E aerogenes is out as it's indole -ve.
S flexneri is out as it's VP -ve.
K pneumonia is out as it's MR -ve.
P vulgaris is out as it's citrate -ve.
S enteriditis is out as it's indole -ve.
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