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Identifying Bacteria

About microscopic forms of life, including Bacteria, Archea, protozoans, algae and fungi. Topics relating to viruses, viroids and prions also belong here.

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Identifying Bacteria

Postby sasha25 » Tue Nov 29, 2011 1:01 am

Hello, I am taking Microbiology 205. We have to figure out the unknowns.. I have 2.. But I am having lots of trouble figuring out what this is.. Please help me..

Gram +
Cell Shape rods and chains
No motility
favorite temp 37
no color on the Agar
it is aerobic
positive for glucose,and mannitol, MR test, and Catalyst
Neg for vp
It is also weak in for starch.

I crossed out many of the choices due to the shape.. Here are the rest of the choices.
Bacillus cereus, Bacillius megaterum, bacillus subtilis, clostridium bejerincki,clostridium sporogenes, mycobacterium phlei (I think this is it), mycobacterium smegmatis.

Please help me. Thank you very much.
sasha25
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Postby marilynkatie » Sun Dec 04, 2011 5:11 pm

Hi, I am tryin to identifying my unknown. I know it is bacillus.
Gram stain- +
motility- +
spore stain- +
acid-fast - negative
capsule - +
optimal temp. 37
facultative anaerobe
all positive for in acid-gluocose,lactose, sucrose, mannitol
nethyl red- +
VP- negative
castalase test- positive
oxidase, hydrogen sulfide, citrate, phenylalanine, indole, urea, are all negative
starch hydrolysis, and gelatine are positive.

I have narrowed it down but I still can't find it.
Please help me and Thank you very much.
marilynkatie
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