Chloroplast

by **monkeymadd** » Mon Nov 28, 2011 6:00 pm

Its a transmission electron micrograph of something in a chloroplast but i can't figure out what it is. Help

by **JackBean** » Mon Nov 28, 2011 8:24 pm

what about size? How do you know it's from chloroplast? It's obviously not inside, so how did you obtain the picture?

by **monkeymadd** » Mon Nov 28, 2011 10:39 pm

Top pic Scale bar = 500 nanometers
botttom pic scale bar = 100 nanometers

I know it is a chloroplast because we isolated the chloroplasts from spinach leaves.

The top pic is the whole mount of a chloroplast taken using the TEM. Dark circular structures can be seen within the chloroplast. bottom pic shows one of these structures from sideways on at higher magnification.
but i don't know what these structures are called.

by **monkeymadd** » Mon Nov 28, 2011 11:10 pm

Are they grana?

by **JackBean** » Tue Nov 29, 2011 1:34 pm

so the whole bunch should be one chloroplast? Seems little bit hydrolysed, isn't it?

by **monkeymadd** » Tue Nov 29, 2011 10:33 pm

yea the top pic is supposed to be a chloroplast.
but I have no idea if it is hydrolysed.

by **JackBean** » Wed Nov 30, 2011 4:27 pm

that doesn't seem like spherical to me

Chloroplast

Chloroplast

Re: Chloroplast

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