Plate count agar.

[ZEGSUS]

What to use instead yeast extract and peptone (http://en.wikipedia.org/wiki/Plate_count_agar).Is it possiblle to use broth and boiled yeast?

[JackBean]

check the composition of both, but I thing it should be interchangeable. However, it might become kind of selective, because some elements may be limiting for some bacteria.

[ZEGSUS]

And do you know a growth medium which I can make it from home materials?

[JackBean]

I'm not microbiologist, but try to look into old microbiology articles or books. Probably something like meat digests or bujouns.

[canalon]

Many broths (what JackBean calls bujons) from home and thing that you have at home can sustain bacterial growth if properly sterilized. Koch was working with potatoes that had been sterilized on the surface then cut with a sterile knife. They could grow colonies on that too.
What you want is to put it in a sterile container, that closes, but not too tightly to let the air in. But you cannot be sure about the selectivity of your medium. Will you be able to grow everything on it, I seriously doubt it. Good luck and be careful what you grow, as many of the wild bacteria can be pathogenic, and having too much of them can actually be dangerous for you, or for those that come in contact with your wastes.

[ZEGSUS]

I will use milk and glucose.I hope that this will be enough.I have a very effective way to sterilize them.

[canalon]

Good luck then. Remember the best way to sterilize is a pressure cooker for 20 minutes after it starts whistling. I am not sure that milk will survive that. And as a matter of fact milk contains a lot of proteins that reduces the availability of iron and will inhibit the growth of some bacteria. Honestly a bit of beef broth with salt adjusted to 5 or 10 g per liter (look at the nutritional info to see how much salt you need to add) and eventually glucose would probably be better.
And you can make it into plates using gelatin (but some bacteria will degrade it) or agar agar (available in some grocery stores)

[ZEGSUS]

But the preservatives in the broth will kill the bacterias.Isnt?

[canalon]

Which preservatives? Many do not have any. dehydration or canning or good enough and there are few preservatives. At least from what I can see in my pantry.

[ZEGSUS]

The broth from the store have a preservatives.

[canalon]

Buy organic.
Make it yourself (yes you can do that): boil cheap cuts of meat in water, and you can even adjust the salt content. No need for vegetables, they are here for taste. Filter at the end and sterilize in a pressure cooker.

[ZEGSUS]

But I feel sick from cooked meat.Is there other way?