How to check if primers will work?

[MissMicropipette]

Hello

I have a DNA extraction product that I want to amplify for archaeal 16S DNA using PCR.

I have the sequence of the archaea I am interested in and a few universal PCR primers.

How can I check (in silico) whether these primers will actually work on amplifying the correct sequences?

I am a total noob so please keep jargon to a low!

thank you!

[JackBean]

if are they complementary to your sequence, the best way ho to find out is to try it quae manu

[aptitude]

On Ubuntu Linux, you can use PerlPrimer.

[JackBean]

you can use Perl on any PC