Login

Join for Free!
114088 members


Forgetting iodine when gram staining

About microscopic forms of life, including Bacteria, Archea, protozoans, algae and fungi. Topics relating to viruses, viroids and prions also belong here.

Moderator: BioTeam

Forgetting iodine when gram staining

Postby SWEngineer » Fri Oct 28, 2011 10:28 am

If we forget to do the iodine step for example when doing gram staining, provided that the microorganism we have is gram positive, what will the colour of the stained microorganism in this case be? Will it be with NO colour or will it be pink-red (the colour of the gram negative)? Why is that?

Thanks.
SWEngineer
Garter
Garter
 
Posts: 34
Joined: Sat May 08, 2010 1:24 pm

Postby canalon » Sat Oct 29, 2011 4:21 pm

What is the role of iodine? (look it up)
What does the secondary coloration do? How discriminant is it?
Patrick

Science has proof without any certainty. Creationists have certainty without
any proof. (Ashley Montague)
User avatar
canalon
Inland Taipan
Inland Taipan
 
Posts: 3909
Joined: Thu Feb 03, 2005 2:46 pm
Location: Canada

Postby Cat » Sat Oct 29, 2011 11:14 pm

"Will it be with NO colour or will it be pink-red (the colour of the gram negative)? " - pink-red

"Why is that?" - look up the function of iodine in gram staining. What happens when it's added to crystal violet?
Cat
King Cobra
King Cobra
 
Posts: 625
Joined: Thu Feb 14, 2008 7:40 pm



Return to Microbiology

Who is online

Users browsing this forum: No registered users and 2 guests

cron