Login

Join for Free!
118822 members


Western Blot

Genetics as it applies to evolution, molecular biology, and medical aspects.

Moderator: BioTeam

Western Blot

Postby hellllo » Wed Aug 31, 2011 12:39 pm

Hello Forum,

please have a look at this image

http://imageshack.us/photo/my-images/98/0bigg.jpg/

There are two proteins A and B in the right column.
Marker B is the protein´s length of healthy people.
But as you can see there are two different markers for this protein (the western Blot was made for one patient).

How can you explain the appearance of two different markers in one patient?

Can you explain with examples how changes of genes can lead to this result?
hellllo
Garter
Garter
 
Posts: 2
Joined: Wed Aug 31, 2011 12:30 pm

Postby JackBean » Wed Aug 31, 2011 2:18 pm

1) why do you post from other username?

2) e.g. heterozygoty, alternative splicing, degradation during sample preparation
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.
User avatar
JackBean
Inland Taipan
Inland Taipan
 
Posts: 5689
Joined: Mon Sep 14, 2009 7:12 pm

Re: Western Blot

Postby hellllo » Wed Aug 31, 2011 3:15 pm

Thank you.

What is about mutations, such as nonsense-mutation?

Marker B refers to the protein Enolase.

Considering heterozygothy is it right to say that the patient inherited the genes. One of his parents has the "healthy" gene and the other not and, therefore, both are expressed (co-dominant expression).
hellllo
Garter
Garter
 
Posts: 2
Joined: Wed Aug 31, 2011 12:30 pm


Re: Western Blot

Postby JackBean » Wed Aug 31, 2011 5:04 pm

hellllo wrote:What is about mutations, such as nonsense-mutation?

Not sure, what means nonsense mutation, but if you have introduced premature STOP, then you would get shorter protein, of course.

hellllo wrote:Considering heterozygothy is it right to say that the patient inherited the genes. One of his parents has the "healthy" gene and the other not and, therefore, both are expressed (co-dominant expression).

Yes
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.
User avatar
JackBean
Inland Taipan
Inland Taipan
 
Posts: 5689
Joined: Mon Sep 14, 2009 7:12 pm

Re: Western Blot

Postby greatmicrobiologist » Fri Sep 02, 2011 11:29 am

hellllo wrote:
What is about mutations, such as nonsense-mutation?


Nonsense mutation is the premature stop of the translation due to point mutation, leading to a stop codon. Thus results in the formation of non-functional protein product.

I am little bit confused. Are you searching for answers or asking us to clarify our understanding :?: :roll:
Saumyadip Sarkar
Student of M.Sc. Microbiology,
GITAM Institute of Science,
GITAM University, AP, India

e-mail: saumyadip.gis@gmail.com

Article Writer
http://www.microbioworld.com/
User avatar
greatmicrobiologist
Garter
Garter
 
Posts: 30
Joined: Thu Aug 25, 2011 7:28 am
Location: Vishakhapatnam, Andhra Pradesh, INDIA

Re: Western Blot

Postby merv » Mon Oct 03, 2011 1:55 am

helllo wrote:What is about mutations, such as nonsense-mutation?


greatmicrobiologist wrote:Nonsense mutation is


<splice the greatmicrobiologist>a point mutation which turns a codon from any of the others into one of the three possible stop codons. This causes

greatmicrobiologist wrote:the premature stop of the translation


<splice the greatmicrobiologist>

greatmicrobiologist wrote:Thus results in the formation of a non-functional protein product.


well, it may still function, many do as they have lots of crap in them they dont need.

greatmicrobiologist wrote:I am little bit confused. Are you searching for answers or asking us to clarify our understanding :?: :roll:


I think she wants you. :lol:
merv
Death Adder
Death Adder
 
Posts: 58
Joined: Sun Oct 02, 2011 2:05 pm


Return to Genetics

Who is online

Users browsing this forum: No registered users and 2 guests