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how to attach dendritic cells for imaging?

Discussion of all aspects of biological molecules, biochemical processes and laboratory procedures in the field.

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how to attach dendritic cells for imaging?

Postby aguy » Wed Aug 03, 2011 7:53 am

Hi, I want to carry out actin staining on human dendritic cells derived from a cell line. The cells are grown in suspension. I am having trouble fixing the cells and subsequent staining because the cells do not attach to a surface (I have tried coating a glass slide with poly L lysine, but it didn't work). Are there any other ways to either attach the cells to a slide or to stain for actin in these cells? I will appreciate any help.

Thanks,
John.
aguy
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