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What happens to free phosphates after DNA elongation in PCR?

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What happens to free phosphates after DNA elongation in PCR?

Postby Melstmu2010 » Thu Jun 23, 2011 8:43 pm

When Taq Polymerase adds free nucleotides to the 3' OH end of template DNA, 2 of the 3 phosphates of the nucleotide are removed (creating the energy necessary to form the phosphodiester bond that attaches the remaining phosphate group to the growing chain). What happens to those 2 phosphates that are removed? How does this effect the other molecules in the PCR solution?
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Postby JackBean » Fri Jun 24, 2011 5:52 am

it's hydrolysed (at least in the cell) and is further metabolised (in the cell). In the PCR tube it may only inhibit the polymerase...
http://www.biolib.cz/en/main/

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