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Urea/EDTA treatment

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Urea/EDTA treatment

Postby Adz795 » Fri Jun 17, 2011 6:13 pm

Q. Urea or EDTA treatment of membranes leads to the dissociation of:
1) Peripheral proteins
2) Integral proteins
3) Cholesterol molecules
4) Phospholipids

I don't have any understanding of Urea/EDTA treatment of membranes. I have never come across it. To answer this question I need to know it. Please tell me a resource where I can find details of this process or explain me please.

Thank you.
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Death Adder
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Postby JackBean » Sat Jun 18, 2011 10:34 am

urea denatures proteins, EDTA is chelator (it binds strongly metal ions), however, to my knowledge, they shouldn't affect lipids

Cis or trans? That's what matters.
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