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Genetic Eng: How do you ID the gene from the protein

Discussion of all aspects of biological molecules, biochemical processes and laboratory procedures in the field.

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Genetic Eng: How do you ID the gene from the protein

Postby nimrod1212 » Sun Jun 05, 2011 6:15 am

Say I have a protein like insulin. I want to find the gene for it, in say the human genome (this is the era before gene banks). Please can someone tell me the steps that get me back to that DNA sequence. i.e. How did Herbert Boyer originally reverse engineer?

I've googled this up and down the yin-yang and looked in various text books (e.g. Lodish et al.). Loads of info about restriction enzymes and ligases and vectors, which I know about. They all jump over the interesting bit at the beginning and simply say, isolate the gene, next... What comes after is the boring tools and cookery, however clever.

Thanks!

Jim
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Postby JackBean » Sun Jun 05, 2011 8:40 am

you need to create either cDNA or gDNA library; you translate piece of your protein into DNA, possibly some part, which is not too much degenerated and make probe from that. Then you mix it together and look, which fragment aligns.
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.
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Postby Skimp210 » Mon Jun 06, 2011 2:00 am

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Postby JackBean » Mon Jun 06, 2011 5:07 am

how can these videos help with his question?
http://www.biolib.cz/en/main/

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