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Positive vs negative control

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Positive vs negative control

Postby Lah » Sat Aug 27, 2005 9:27 pm

Does anyone have an acceptable definition for Positive vs negative control?
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Postby mith » Sun Aug 28, 2005 1:17 am

acceptable?
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Postby Poison » Sun Aug 28, 2005 4:14 pm

maybe he/she meant "understandable"...
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find a definition

Postby ccljboy » Tue Aug 30, 2005 1:21 am

try wikipedia, or this sites dictionary(but i didnt find it there)
knowledge gives us the right to make opinoins.
the opinoins of a fool are worthless, and make those that hear them foolish.
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Postby Poison » Tue Aug 30, 2005 3:53 pm

Edit: This was a message that I wrote due to stupidity. :?
Last edited by Poison on Tue Aug 30, 2005 4:13 pm, edited 1 time in total.
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Postby canalon » Tue Aug 30, 2005 4:08 pm

In an experiment the positive control is an experiment whose result is already known to be positive, it is used to check that nothing went wrong during the procedure. For a PCR detection test it would typically be the amplification of a sample known to yield the correct amplicon.
The negative control is an experiment done with a sample/mock sample that should normally yield a negative result. It allows to check for contamination of the reagents or artifacts that would give false positive. Typically in a PCR that would mean running a PCR reaction with water as a template.

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Postby Poison » Tue Aug 30, 2005 4:12 pm

It's really time for me to start practising english again. :shock: I can't believe it!!! I'm thinking things in my language. That's the worst thing that can happen while using a foreign language. :oops:
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Postby MrMistery » Wed Aug 31, 2005 9:47 pm

I don't understand what you mean, poison...
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Postby Poison » Thu Sep 01, 2005 2:07 pm

Because I edited the post. I got the question wrong. I mean I thought it in my language, but you know, this is english...
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Re: Positive vs negative control

Postby kid » Thu Sep 04, 2008 1:35 am

i like canalon's answer; it is useful. i might need your help though out the learning process--in my biology classes. Every one please don't hesitate to correct my mistakes. this is my first semester in biology class.
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