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Urgent help preparing cycloheximide (CHX)

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Urgent help preparing cycloheximide (CHX)

Postby bravebeaker » Mon May 23, 2011 7:22 am

Hi everyone!
I came across the preparation of cycloheximide (CHX). I found the powder stored in the freezer but the vial have no information.
I googled cycloheximide and the way I could prepare it but I only found the mol. wt. 281.35 g/mol and solubility in ethanol.
does it mean I can dissolve it in 100% ethanol?
and if i want to always add 300 uM to 50 ml solution, how can I prepare the stock solution?
thanks everybody!
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Re: Urgent help preparing cycloheximide (CHX)

Postby JackBean » Mon May 23, 2011 8:08 am

yes, that would mean, that you can dissolve it in pure ethanol. But here they write, it's soluble in MeOH, diethylether and acetone. And also sligthly in water.

However, regarding your second question, you cannot add 300 uM of something, because it's the unit of concentration, so you can either add 300 umol or some volume of 300 uM solution or some volume of some solution, so that you will have 300 uM concentration. What's your choice?
http://www.biolib.cz/en/main/

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Postby bravebeaker » Mon May 23, 2011 9:16 am

Thanks for the quick reply!
well.. I need the final concentration to be 300 uM in the 50 ml solution.
But can you elaborate either ways please?
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Postby JackBean » Mon May 23, 2011 10:06 am

well, then it depends on how concentrated stock solution you will be able to make ;)
E.g. If you had 3 mM solution, you will dilute it 10-times, thus you need to add 1 ml of the stock. Ideally to 49 ml of your solution, thus it's better to make your stock solution as concentrated as possible, e.g. if you had 300 mM, you will need to dilute it 1000-times and thus you will add 50 ul and then it doesn't make much difference, whether you add it to 50 ml or 49.95 ml ;)
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Postby bravebeaker » Mon May 23, 2011 11:08 am

Hi again.
To apply what you just said:
300mM is 0.3M concentration.
to prepare a liter--> 0.3 x 281.35 = 84.41 g
for 10 ml of CHX solution --> dissolve 0.844 g in 100%
take 50 ul of CHX solution and add it to the 50 ml solution and this way I will obtain 300 uM final concentration?
Hope this is correct!
Thanks alot again.




So you mean that I can prepare 300 mM CHX solution by dissolving
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Postby JackBean » Mon May 23, 2011 11:20 am

yeah, that should be OK.

However, question is, whether will you be able to get such concentrated solution.
Another reason, why to use as much concentrated solution as possible is to avoid addition of large amount of organic solvents to your system.
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Postby bravebeaker » Mon May 23, 2011 12:06 pm

I got it now! Thank you alot jackbean :)
I will see how much powder I have of CHX and according to it I will act.
And you mentioned a very important idea..should not use solvant alot.
Thanks again :)
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