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Oxidative Phosphorylation

Discussion of all aspects of biological molecules, biochemical processes and laboratory procedures in the field.

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Oxidative Phosphorylation

Postby uer23 » Wed Mar 16, 2011 8:16 pm

1) Using the data below:


a) which compound increases oxygen uptake by mitochondria in the presence of succinate ?
b) which compound blocks complex I but has no effect on electrons flowing from succinate ?
c) which compound blocks electron flow to complex 2 ?



In the graph above where oxygen uptake by mitochondria in the presence of glutamate as a substrate, which compound inhibits the flow of electrons into complex 1 ?

So far I've concluded:

1.a) Rotenone
1.b) Rotenone
1.c) Antimycin

Can someone just clarify these and help me on question 2. Thanks
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Postby JackBean » Mon Mar 28, 2011 7:16 am

I belive, if there is supposed to be increased oxygen, the electric potential should drop faster ;)

2. similarly as in 1, where have you found it. If you block the flow, the potential should be probably the same, rigth?

Cis or trans? That's what matters.
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