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Isothermal mRNA amplification on solid state?

Discussion of all aspects of biological molecules, biochemical processes and laboratory procedures in the field.

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Isothermal mRNA amplification on solid state?

Postby ttrndy » Mon Mar 07, 2011 6:22 am

Hi Everyone

I’m currently work on cancer cell mutation analysis. I’m going to amplify the mRNA for further research. I need an amplification techniques that can amplify mRNA in iso-thermal condition,primer attached on the solid surface. Are there anyone else know about that techniques?

I also take a look at RCA and RAM but it seems unsatify my purpose because i need to amplify the mRNA not amplify the signal. I hope to receive your advices soon.

My email: tranduy.bio@gmail.com

All the best
ttrndy
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