Protein Localization

[rosegarden]

Hey guys, so I'm given a long strand of protein (shown below). It is predicted to be nuclear localized but how can I know that it's nuclear localized just by looking at its primary sequence? I mean we can probably do some protein tagging to localize it experimentally but I have no idea how the prediction part comes about. Thanks in advance for any help.

mdkvlnreeslqlmdllglersawgniplmrkaylkkckefhpdkggdeekmkkmntlyk
kmedgvkyahqpdfggfwdateiptygtdeweqwwnafneenlfcseempssddeatads
qhstppkkkrkvedpkdfpsellsflshavfsnrtlacfaiyttkekaallykkimekys
vtfisrhnsynhnilffltphrhrvsainnyaqklctfsflickgvnkeylmysaltrdp
fsvieeslpgglkehdfnpeeaeetkqvswklvteyametkcddvllllgmylefqysfe
mclkcikkeqpshykyhekhyanaaifadsknqkticqqavdtvlakkrvdslqltreqm
ltnrfndlldrmdimfgstgsadieewmagvawlhcllpkmdsvvydflkcmvynipkkr
ywlfkgpidsgkttlaaallelcggkalnvnlpldrlnfelgvaidqflvvfedvkgtgg
esrdlpsgqginnldnlrdyldgsvkvnlekkhlnkrtqifppgivtmneysvpktlqar
fvkqidfrpkdylkhclersefllekriiqsgialllmliwyrpvaefaqsiqsrivewk
erldkefslsvyqkmkfnvamgigvldwlrnsddddedsqenadknedggeknmedsghe
tgidsqsqgsfqapqssqsvhdhnqpyhicrgftcfkkpptpppepet

[canalon]

try some of those

http://www.geneinfinity.org/sp/sp_proteinloc.html

[rosegarden]

I have used predictor tools but I want to know how to predict localization just by looking at the primary sequence.

[JackBean]

but that's what the predictors do, they look on the primary structure and look for specific patterns, which could mean localisation into any organelle

[rosegarden]

does this have something to do with locating a part of the main sequence called the nuclear localization signal with indicates nuclear localization? and if so what is the sequence of the nuclear localization signal?

[JackBean]

http://www.lmgtfy.com/?q=nuclear+locali ... signal&l=1