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Nanodrop

Discussion of all aspects of biological molecules, biochemical processes and laboratory procedures in the field.

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Nanodrop

Postby Nmr » Mon Feb 14, 2011 4:00 pm

Hi my dear friend...
I am a little bit confuse about nanodrop and it function.. if anyone here has idea about it plz tell me.

what is the nanodrop machine and what are its applications?
how nanodrop is work?

best regards
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Re: Nanodrop

Postby jonmoulton » Mon Feb 14, 2011 4:17 pm

A nanodrop is a spectrometer which passes a beam of narrow-band (near-monochromatic) light through a droplet of water and measures the intensity of light transmitted through the sample. More traditional spectrophotometers use cuvettes to contain the sample and generally require considerably more volume of the analyte solution. The advantage of the nanodrop system is that by using only a drop of analyte solution, it conserves sample.
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Postby Kezzer » Sat Feb 19, 2011 2:04 pm

^^^^Agreed!

Whenever I've used Nanodrop it has been to quantify the concentration of DNA in a solution.
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Postby adihutama » Fri Mar 04, 2011 7:37 am

yes, and RNA as well

it got choices in its software of what material that youd like to measure at the moment.
It can even perform Bradford assay, well of course just the reading part though
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