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2D gel IEF equilibration buffer

Discussion of all aspects of biological molecules, biochemical processes and laboratory procedures in the field.

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2D gel IEF equilibration buffer

Postby Hannah2008 » Mon Feb 07, 2011 7:41 pm

Hi guys,
I left my isoelectric focusing (IEF) equilibration buffer out at RT over the weekend. Is it still ok to use?
Thanks,
Hannah
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Postby JackBean » Mon Feb 07, 2011 7:45 pm

it should be fine.

You have there urea, rigth? That would crystalize in refrigerator anyway.
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.
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Postby Hannah2008 » Mon Feb 07, 2011 7:52 pm

I'm not sure why, but these references say to not leave urea buffers at room temperature for extended periods of time.
http://onlinelibrary.wiley.com/doi/10.1 ... 75.oth/pdf
www.hoeferinc.com/downloads/IEF100_IMA0.pdf
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