Join for Free!
121439 members

Differentially Label

Discussion of all aspects of biological molecules, biochemical processes and laboratory procedures in the field.

Moderator: BioTeam

Differentially Label

Postby chengieteo » Sat Feb 05, 2011 3:19 am

Hi all,

I'm quite new in Molecular biology and wish to know more. I saw this two question not very sure how we can do it.

How can we differentially label (make different) DNA and RNA?
How can we differentially label (make different) DNA/RNA and protein?

Hope that someone can assist me in this. Thanks in advance =)

Posts: 1
Joined: Sat Feb 05, 2011 3:16 am

Postby JackBean » Sat Feb 05, 2011 10:01 am

for labeling NAs you can use e.g. Cy3 and Cy5 or plenty fluorescent colors used in qPCR such as TaqMan and many others.

And I think that Cy3 and Cy5 can be used even for proteins. For some other labels look for differential 2D electrophoresis.

Cis or trans? That's what matters.
User avatar
Inland Taipan
Inland Taipan
Posts: 5694
Joined: Mon Sep 14, 2009 7:12 pm

Re: Differentially Label

Postby peyem » Tue Feb 08, 2011 5:36 am

For RNA and DNA, is it the uracil in RNA and the thymine in DNA that will produce different fluorescent colours?
Posts: 1
Joined: Tue Feb 08, 2011 5:34 am

Postby canalon » Wed Feb 09, 2011 2:29 am

There are countless fluorescent markers who have different physical properties and are able to give different colors.
T and U are not fluorescent by themselves, but if a fluorescent moiety is attached to them (obviously with different spectral properties) they will be easy to separate.

Handy list of different fluorophores here:

One thing they have in common though is that they are quite expensive.

Science has proof without any certainty. Creationists have certainty without
any proof. (Ashley Montague)
User avatar
Inland Taipan
Inland Taipan
Posts: 3909
Joined: Thu Feb 03, 2005 2:46 pm
Location: Canada

Return to Molecular Biology

Who is online

Users browsing this forum: No registered users and 1 guest