Differentially Label

[chengieteo]

Hi all,

I'm quite new in Molecular biology and wish to know more. I saw this two question not very sure how we can do it.

How can we differentially label (make different) DNA and RNA?
How can we differentially label (make different) DNA/RNA and protein?

Hope that someone can assist me in this. Thanks in advance =)

Regards,
Cheng

[JackBean]

for labeling NAs you can use e.g. Cy3 and Cy5 or plenty fluorescent colors used in qPCR such as TaqMan and many others.

And I think that Cy3 and Cy5 can be used even for proteins. For some other labels look for differential 2D electrophoresis.

[peyem]

For RNA and DNA, is it the uracil in RNA and the thymine in DNA that will produce different fluorescent colours?

[canalon]

There are countless fluorescent markers who have different physical properties and are able to give different colors.
T and U are not fluorescent by themselves, but if a fluorescent moiety is attached to them (obviously with different spectral properties) they will be easy to separate.

Handy list of different fluorophores here:
http://en.wikipedia.org/wiki/Fluorophore

One thing they have in common though is that they are quite expensive.