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MTT at early timepoints after cell seeding

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MTT at early timepoints after cell seeding

Postby outremer » Tue Jan 25, 2011 12:17 pm

Hi, I´m new to this field. Is it a good idea to quantify amount of adherent cells on titanium at early time-points(30 min, 1h ...) with MTT assay?

One thought I had was that newly trypsinized cells that are seeded on a surface are not very happy, which perhaps affect their metabolic activity - measured by MTT.

So my question, is MTT a suitable technique for newly trypsinized cells adhered on a surface for 30 min?

Grateful for any help!

/Erik
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