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simple epitope

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simple epitope

Postby derChronostat » Tue Jan 18, 2011 9:48 am

Hi,

I couldn´t rally figure out where to post this question.
Does anybody know an antibody that recognizes a very simple epitope - like a single loop? For example a peptide-antibody or an antibody binding to a single "site" on a protein.
Most of the antibodies recognize a context of several subepitopes which is too complicated for my approach.

Thanks in advance,

Chris
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Postby JackBean » Tue Jan 18, 2011 11:06 am

you should be able to to raise antibodies only against your epitope (understand - use only that epitope for imunization), or raise polyclonal antibodies and lok, which one will best bind your epitope
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.
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Re: simple epitope

Postby derChronostat » Tue Jan 18, 2011 11:16 am

Hi,

thanks for your reply.

this is not what the thing is about. I need something like a positive control - a known antibody known to bind a very simple epitope (best would be a loop - hence a nonlinear peptide or protein-fragment) which can be used in in-vitro experiments.

Cheers,
Chris
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Postby JackBean » Tue Jan 18, 2011 11:45 am

I'm sorry, I don't understand.
http://www.biolib.cz/en/main/

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Postby Darby » Tue Jan 18, 2011 8:50 pm

I learned that there is a lower limit to the size of any potential epitope - is that what this is about-?
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Postby canalon » Wed Jan 19, 2011 1:44 am

Epitopes are more than just a loop or any other shape since they include the shape of each aa in the sequence that makes that loop (or other).
I am not sure what you want to do but you can order specific peptides made by a number of companies, and some will also sell Ab specifically targeting those peptides. Would that be what you are looking for?
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