Preparing primary cell cultures?

[dmitrip]

Hello,

I am learning about preparing primary cell cultures and my textbook mentions that to prepare tissue cells, the cell-cell and cell-matrix interactions must be broken using methods like mechanical fragmentation, trypsin and EDTA. This procedure separates the cell.

What I am wondering is why do the cell-cell and cell-matrix interactions must be broken in the first place?

Thank you very much for any help!

[JackBean]

because the cell cultures are usually liquid and if the interactions were not broken, it could not be liquefied.