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Preparing primary cell cultures?

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Preparing primary cell cultures?

Postby dmitrip » Tue Jan 04, 2011 6:58 pm


I am learning about preparing primary cell cultures and my textbook mentions that to prepare tissue cells, the cell-cell and cell-matrix interactions must be broken using methods like mechanical fragmentation, trypsin and EDTA. This procedure separates the cell.

What I am wondering is why do the cell-cell and cell-matrix interactions must be broken in the first place?

Thank you very much for any help!
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Postby JackBean » Mon Nov 28, 2011 4:22 pm

because the cell cultures are usually liquid and if the interactions were not broken, it could not be liquefied.

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