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Binding curves and non-specific samples

Discussion of all aspects of biological molecules, biochemical processes and laboratory procedures in the field.

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Binding curves and non-specific samples

Postby fcs » Fri Dec 24, 2010 9:02 pm

Hi,

When trying to plot binding curves such as competitive binding curves, is the non-specific binding (NSB) sample required?
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Postby JackBean » Fri Dec 24, 2010 9:18 pm

I think it's good, because you can say "OK, if there is nonspecific binding e.g. by albumin (or whatever), it's just 3%, while with the same amount of my protein it is 20%, so it's probably specific" ;)
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.
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