IC50

[DivyaG]

hey IC50

[andresgutierrez]

Hello,

You should plot a graph - X axis - concentration of drug and Y- axis - cell viability - ( Abs ( with drug) / Abs(control without drug addition ) *100%

So you can estimate Ic 50 as a concentration of drug at 50% position on Y axis.

Andres

[JackBean]

if you're talking about enzymes, you're looking for K_i, not IC50

there are several ways how to determine this value. You can either use simple v (speed of reaction) on [S] (as Michaelis-Menten graph), or Lineweaver-Burg (1/v on 1/[S]) or plenty of others (e.g. Dixon's, Hanes-Wolf, Wolf-Augustinson-Hoffsteeh, Hunter-Dowson etc)